Journal: Science Advances

Article Title: A plant-specific cytochrome b 5 –like protein is essential for phytosterol biosynthesis

doi: 10.1126/sciadv.ady1719

Figure Lengend Snippet: CB5 and CB5LP homologs of Viridiplantae and Red algae were obtained using AtCB5D and AtCB5LP as queries via BLASTP search against Phytozome ( C. reinhardtii , V. carteri , M. polymorpha , P. patens , S. moellendorffii , C. richardii , A. trichopoda , O. sativa , and P. trichocarpa ), FernBase ( S. cucullata ), Genbank ( P. purpureum , G. domingensis , R. marinus , C. caldarium , G. chorda , and C. merolae ), ONEKP ( C. vulgaris , P. radiata , and G. biloba ), and Solanaceae Genomics Network ( P. axillaris ). Metazoa, Fungi, and Bacteria CB5 and CB5LP homologs were obtained using AtCB5LP as query via BLASTP search against Clustered nr (nr_clustered) database. Maximum likelihood tree was constructed with full-length proteins using IQ-tree2 algorithm (Q.pfam+R6 model selected by ModelFinder, 1000 SH-aLRT tests, and 1000 ultrafast bootstraps). Only major bifurcate bootstrap support values are marked on the main branches. Bacterial proteins with and without a single TM domain are symbolized with stars and dots, respectively. The scale bar indicates average number of amino acid substitutions per site.

Article Snippet: Constructs were transformed into a yeast strain deficient in its endogenous CB5 ( cyb5 , MAT-a, Transomic), and positive colonies were selected on auxotrophic yeast medium lacking uracil.

Techniques: Algae, Bacteria, Construct

Journal: International journal of molecular sciences

Article Title: Two-Step Cell Death Induction by the New 2-Arachidonoyl Glycerol Analog and Its Modulation by Lysophosphatidylinositol in Human Breast Cancer Cells.

doi: 10.3390/ijms26020820

Figure Lengend Snippet: Figure 6. The effect of receptor blockers on the effect of 2-ADFP (EC50-EC70) combination with LPI (5 µM) on breast cancer cell line viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, PSB CB5 (3 µM); and TRPV1, capsazepine (5 µM). Incubation time 72 h, resazurin test, mean ± standard error (N = 4 experiments). (A) MCF-10A, (B) MCF-7, (C) BT-474, (D) SK-BR-3, (E) BT-20, and (F) MDA-MB- 231. *, a statistically significant difference from 2-ADFP alone, p ≤0.05, ANOVA with Holm–Sidak post-test.

Article Snippet: SR 144028, PSB CB5, ML-184, ML-193, capsazepine, and LPI was from Tocris Biosci- ence, Bristol, UK.

Techniques: Incubation